Fig. 6.

Chondrosarcoma cells sufficiently express SLC22A1 for metformin to completely inhibit mitochondrial respiration. a All chondrosarcoma cell lines express SLC22A1. b SLC22A2 is only expressed by L2975. c Three out of ten chondrosarcoma cell lines express SLC22A3. d, e The impact of 24 h treatment with 5 mM metformin on the Oxygen Consumption Rate (OCR)(D) and extracellular acidification rate (ECAR)(E) of JJ012, SW1353 and CH2879 was measured by Seahorse experiments. Metformin completely blocks mitochondrial respiration, which is only accompanied by a small increase in glycolysis