Figure 2. Protocol schematic.
Subjects underwent two visits to the Clinical Research Center. During the first visit, blood was drawn every 10 minutes to measure luteinizing hormone (LH) at baseline (from 2–8 am), after an intravenous bolus of kisspeptin, and after an intravenous bolus of gonadotropin-releasing hormone (GnRH). Subjects then underwent pituitary “priming” with exogenous GnRH and then returned for a second visit to measure LH after boluses of kisspeptin and GnRH. IV, intravenous; SC, subcutaneous; q2h, every 2 hours.