Figure 5. NRDC regulates apoptosis and chemosensitivity though p53.

(A) HCT116 cells, in which NRDC and p53 were concomitantly knocked down, were treated with or without 5-fluorouracil (5-FU, 5 μM) and oxaliplatin (L-OHP, 1 μM) for 48 hours and harvested for Western blotting. The effects of NRDC knockdown were cancelled by concomitant p53 knockdown. (B) The number of viable HCT116 cells was quantified using the WST-8 assay. All data represent means ± SE of 4 independent experiments with duodecuplicate samples. (C) qRT-PCR analysis showed that the mRNA levels of p53 were not affected by the NRDC status. All data represent means ± SE of 3 independent experiments with duplicate samples. (D) qRT-PCR analysis showed that p53 mRNA was slightly but significantly downregulated in Apc^Min; Villin-cre; Nrdc^fl/fl mice. All data represent means ± SE of 4 independent experiments. *P < 0.05 by unpaired 2-tailed Student’s t test.