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. 2018 Apr 16;74(Pt 5):277–282. doi: 10.1107/S2053230X18004557

Table 1. Crystallization conditions.

Method Hanging-drop vapour diffusion
Plate type 24-well plates
Temperature (°C) 4
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 20 mM Tris pH 7.5, 0.1 M NaCl
Composition of reservoir solution 0.1 M MES pH 5.5–6.5, 2.8 M ammonium sulfate
Volume and ratio of drop 2 µl:2 µl
Volume of reservoir (ml) 1