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. 2018 Apr 16;74(Pt 5):294–299. doi: 10.1107/S2053230X1800506X

Table 2. Crystallization.

Method Sitting-drop vapor diffusion
Plate type 96-well Compact 300, Rigaku
Temperature (K) 290
Protein concentration (mg ml−1) 20 (diluted from 58.13)
Buffer composition of protein solution 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
Composition of reservoir solution JCSG-plus condition C3: 20%(w/v) PEG 3350, 200 mM ammonium nitrate
Volume and ratio of drop 0.4 µl:0.4 µl
Volume of reservoir (µl) 80