Table 2. Crystallization.
Method | Sitting-drop vapor diffusion |
Plate type | 96-well Compact 300, Rigaku |
Temperature (K) | 290 |
Protein concentration (mg ml−1) | 20 (diluted from 58.13) |
Buffer composition of protein solution | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
Composition of reservoir solution | JCSG-plus condition C3: 20%(w/v) PEG 3350, 200 mM ammonium nitrate |
Volume and ratio of drop | 0.4 µl:0.4 µl |
Volume of reservoir (µl) | 80 |