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Fig. 5. Specificity analysis of HeLa cells. (a) Confocal images of HeLa cells treated with different 0.2 nM nanomachines (S2/R1/A6-AuNP, S2/R1/A7-AuNP, S2/R1/A8-AuNP and S2/R1/A9-AuNP) for 2 h. (b) Mean fluorescence values of the tested nanomachines in HeLa cells via analysis of NIS-elements. Error bars represent the standard deviation from n = 3 experiments. (c) Real-time fluorescence intensity of different 0.1 nM nanomachines (S2/R1/A6-AuNP, S2/R1/A7-AuNP, S2/R1/A8-AuNP and S2/R1/A9-AuNP) in response to 5 nM T1.