Figure 8.

BL-induced accumulation of RNA derived from transgenic constructs containing the truncated Lhcb1*4 promoter −95 to +2. Six-day-old dark-grown transgenic Arabidopsis seedlings containing a truncated pea Lhcb1*4 promoter extending from −95 on the upstream end to +2 on the downstream end (where +1 represents the site of transcriptional initiation) fused to GUS were irradiated with a single pulse of BL with a total fluence of 10² μmol m⁻² or 10⁴ μmol m⁻² or were given no light (D). RNA was prepared 2 h after the light treatment and analyzed by northern blotting. The resulting blots were probed simultaneously for GUS and Lhcb RNA, representing expression of the transgene and the endogenous Arabidopsis Lhcb, respectively.