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. 2018 Feb 16;55(5):298–306. doi: 10.1136/jmedgenet-2017-104954

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Immunofluorescence analysis of Ifnlr1 expression in the inner ear of P30 mice. (A) Schematised localisation of Ifnlr1 in the cochlea and spiral ganglion. Staining appears in IHC, OHC, ISC, IPC, OPC, SC, SP, SG, CN, VM, BM and ESCs. The blank cochlea diagram is from the hereditary hearing loss homepage (http://hereditaryhearingloss.org). (B) Overview of Ifnlr1 expression (green) in the cochlea. (C) Details of the organ of Corti showing that Ifnlr1 is highly expressed in the cytoplasm and nuclei of most cells with the exception of the OHCs, which exhibit Ifnlr1 expression only in cytoplasm and not in the nuclei. (D–E) Details of the cochlear nerve (D) and the spiral ganglion (E), in which the structures are clearly stained by antibody directed against the heavy chain of neurofilament (NF200). The merged picture shows partial overlap of Ifnlr1 and NF200 in the nerve fibres and the SG cells. (F) Details of the crista ampullaris showing staining in the vestibular hair cells, supporting cells, stereocilia and nerve fibres. (G–J) Expression of Ifnlr1 (green) in optical section of whole mount of Corti. (G) Stereocilia level. Staining is present in stereocilia of IHC and OHC. (H) Level of cuticular plate of IHC. Staining is observed at the cytoplasm of OHC and also in cuticular plate of IHC. (I) Level above OHC and IHC nuclei. Staining is observed in IHC and OHC cytoplasm. (J) Level of OHC and IHC nuclei. Staining is observed in IHC nuclei, and OHC nuclei are not stained. Nuclei were visualised with DAPI (blue) and F-actin with phalloidin (red). BM, basilar membrane; CN, cochlear nerve; ESCs, external sulcus cells; IHC, inner hair cells; IPC, inner pillar cells; ISC, inner sulcus cells; OHC, outer hair cells; OPC, outer pillar cells; SC, support cells; SG, spiral ganglion; SP, spiral ligament; VM, vestibular membrane.