Figure 1.

Laser spot titration and distribution. (A) Laser spots were titrated in the peripheral murine retina (red box) starting from above threshold (from left to right: first white burn) to barely visible lesions (third barely visible burn). The red spot is the aiming beam. TS-R laser treatment was carried out further centrally (red line). All treatment spots were funduscopically invisible. In OCT images, titration lesions were visible (B), and TS-R treated areas showed no anatomical alterations in OCT (C). TS-R treatment spots were distributed uniformly across an optic nerve centered approximately 50° field of view at two-spot interspot spacing, sparing optic nerve disc and retinal vessels (D).