Table 1.

Selected O-GlcNAc Enriched Proteins Identified in Glycoproteomics Analysis with High Confidence^a

protein	gene	Det	ref	Loc	function	Phos	pathways
plastin-2	Lcp1	E	-	Cyt	other	Y	-
40S ribosomal protein S23	Rps23	E	36	Cyt	translation regulator	-	EIF2 signaling; mTOR signaling; eIF4 and p70S6K signaling
cell division cycle and apoptosis regulator protein 1	Ccar1	M	24, 36	Nuc	transcription regulator	Y	-
cathepsin B	Ctsb	M	36	Cyt	peptidase	-	autophagy; inflammasome pathway; phagosome maturation
hexokinase-1	Hk1	M	-	Cyt	kinase	-	GDP-glucose biosynthesis; glucose and glucose-1-phosphate degradation
E3 ubiquitin-protein ligase HUWE1	Huwe1	M	36–38	Nuc	transcription regulator	Y	-
DNA mismatch repair protein Msh6	Msh6	M	-	Nuc	enzyme	Y	colorectal cancer metastasis signaling; mismatch repair in eukaryotes
nuclear pore complex protein Nup214	Nup214	M	24, 36	Nuc	transporter	Y	-
nucleoporin p58/p45	Nup58	M	24, 38, 39			-	-
phosphatidylinositol-binding clathrin assembly protein	Picalm	M	24, 36, 38	Cyt	other	Y	clathrin-mediated endocytosis signaling
pleckstrin homology domain- containing, family A member 5	Plekha5	M	38	Cyt	other	-	-
protein phosphatase 1 regulatory subunit 12A	Ppp1r12a	M	24, 36, 38	Cyt	phosphatase	Y	CCR3 signaling in eosinophils; chemokine signaling; ERK/MAPK signaling; HIPPO signaling
RNA-binding protein 27	Rbm27	M	24, 36, 38	Nuc	other	Y	-
deoxynucleoside triphosphate triphosphohydrolase SAMHD1	Samhd1	M	36	Nuc	enzyme	Y	-
SAP30-binding protein	Sap30bp	M	24, 36, 38	Nuc	transcription regulator	Y	-
splicing factor 1	Sf1	M	24, 36	Nuc	other	Y	-
Transcription factor Sp1	Sp1	M	36	Nuc	transcription regulator	Y	ErbB2-ErbB3 signaling; estrogen-dependent breast cancer signaling; IL-10 signaling
spectrin beta chain, nonerythrocytic 1	Sptbn1	M	24, 36, 40	P.M.	other	-	sertoli cell-sertoli cell junction signaling
transcription initiation factor TFIID subunit 6	Taf6	M	24, 36	Nuc	transcription regulator	Y	assembly of RNA polymerase II complex; receptor signaling
ubiquitin-associated protein 2	Ubap2	M	36, 38	Cyt	other	Y	-
ubiquilin-2	Ubqln2	M	36	Nuc	other	Y	-
fructose-bisphosphate aldolase A	Aldoa	D	36, 38	Cyt	enzyme	Y	gluconeogenesis I; glycolysis I; sucrose degradation V(mammalian)
bifunctional glutamate/proline- tRNA ligase	Eprs	D	24, 36	Cyt	enzyme	Y	tRNA Charging
fermitin family homologue 3	Fermt3	D	-	Cyt	enzyme	Y	-
guanine nucleotide-binding protein G(i) subunit alpha-2	Gnai2	D	38	P.M.	enzyme	-	CCR5 signaling; chemokine signaling; IL-8 signaling; leukocyte extravasation signaling;
host cell factor 1	Hcfc1	D	24, 36–40	Nuc	transcription regulator	Y	-
histone H1.3	Hist1h1d	D	36	Nuc	other	Y	granzyme A signaling; protein kinase a signaling
heat shock protein HSP 90-alpha	Hsp90aa1	D	36, 37	Cyt	enzyme	Y	androgen signaling; glucocorticoid receptor signaling; HIF1α signaling; PI3K/AKT signaling
Ras GTPase-activating-like protein IQGAP1	Iqgap1	D	24, 36	Cyt	other	Y	Cdc42 signaling; IL-8 signaling; ignaling by Rho family GTPases
protein disulfide-isomerase	P4hb	D	-	Cyt	enzyme	-	hypoxia signaling in the cardiovascular system; role of tissue factor in cancer; unfolded protein response
proliferation-associated protein 2G4	Pa2g4	D	36	Nuc	transcription regulator	Y	cell cycle: G1/S checkpoint regulation; cyclins and cell cycle regulation
polyadenylate-binding protein 1	Pabpc1	D	36, 38	Cyt	translation regulator	Y	antiproliferative role of TOB in T cell signaling; regulation of eIF4 and p70S6K signaling
programmed cell death 6- interacting protein	Pdcd6ip	D	24, 36	Cyt	other	Y	14-3-3-mediated signaling; mechanisms of viral exit from host cells
splicing factor, proline- and glutamine-rich	Sfpq	D	24, 36, 38	Nuc	other	Y	-
TAR DNA-binding protein 43	Tardbp	D	36	Nuc	transcription regulator	Y	-
valine-tRNA ligase	Vars	D	36	Cyt	enzyme	Y	tRNA charging
14-3-3 protein theta	Ywhaq	D	24, 36	Cyt	other	Y	cell cycle: G2/M DNA damage checkpoint regulation; Myc mediated apoptosis signaling
serine-tRNA ligase, cytoplasmic	Sars	D	-	Cyt	enzyme	Y	selenocysteine biosynthesis; tRNA charging

^aDet (detection): E = effector (p < 0.05), M = memory (p < 0.05), D = detected. ref (reported references). Loc (cellular location): Nuc = nucleus, Cyt = cytoplasm, P.M. = plasma membrane. Phos (known phosphorylation): Y = yes, N = no.