| Item | Amount | Final Concentration |
| 1x PBS | 50 mL | 1x |
| BSA | 1.5g | 3% (w/v) |
| Horse Serum | 5 mL | 10% (v/v) |
| 10% detergent (see Table of Materials) in PBS | 250 μL | 0.05% (v/v) |
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