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. 2018 May 2;13(5):e0195893. doi: 10.1371/journal.pone.0195893

Fig 1. Dysregulation of TNF and wnt-signaling in A20 knockout cell lines.

Fig 1

A) Wild-type (WT 293) and A20 knockout 293 (A20-/- 293) cells were stimulated with TNF for 4 hours. Lysates were then blotted for A20 or GAPDH as a loading control. B) Wild-type (WT) RKO and A20 knockout (A20-/-) RKO cells were stimulated with TNF for 4 hours. Lysates were then blotted for A20 or GAPDH as a loading control. C) Wild-type (WT RKO) or knockout RKO (KO RKO) were transfected with an NFkB-luciferase reporter and then stimulated with TNF for 8 hours. Luciferase activity was measured and normalized to Renilla luciferase. D) Wild-type (WT RKO) or knockout RKO (KO RKO) were transfected with a TCF4-luciferase reporter and then stimulated with wnt3a for 8 hours. Luciferase activity was measured and normalized to Renilla luciferase. E) A20-/- 293 cells were transfected with a plasmid expressing wild-type A20 in increasing concentrations along with a TCF4-luciferase reporter. Cells were stimulated with wnt3a for 8 hours and luciferase-reporter activity measured and normalized to Renilla luciferase. F) WT RKO or A20-/- RKO cells were transfected with a TCF4-luciferase reporter and then stimulated with wnt5a for 8 hours. Luciferase activity was measured and normalized to Renilla luciferase. * = p < 0.05, ** = p < 0.01. Each panel is representative of at least three independent experiments.