Fig. 6.

Dkk3-induced FoxO3-dependent recruitment of β-catenin on the core promoters of Fbxo32 and Trim63. a Schematic illustration of the core promoter structures of Fbxo32 and Trim63. b ChIP analysis of RNA pol II, FoxO3, β-catenin, and Tcf3 on Fbxo32 and Trim63 promoters in primary myotubes treated with Dkk3 or control protein. Error bars indicated s.d. and were based on four independent experiments. * indicated p < 0.1. ** indicated p < 0.01. *** indicated p < 0.001. NS indicated no significant changes. c ChIP analysis of RNA pol II, FoxO3, β-catenin, and Tcf3 on core promoters of Fbxo32 and Trim63 in muscle tissues isolated from young adult (3 months) or aged (20 months) mice. Error bars indicated s.d. and were based on five independent experiments. * indicated p < 0.1. ** indicated p < 0.01. *** indicated p < 0.001. NS indicated no significant changes. d Nuclear extracts prepared from myotubes treated with Dkk3 or control protein were subjected to immunoprecipitation with FoxO3 antibody. The immunoprecipitated proteins were subjected to immunoblotting with β-catenin and Tcf3. Lane 1 and 2 were 10% of input nuclear extracts. Lane 3 and 4 represented immunoprecipitation with FoxO3 antibody. e ChIP analysis of RNA pol II, FoxO3, β-catenin, and Tcf3 on the core promoters of Fbxo32 and Trim63 in FoxO3 null TA muscles with or without Dkk3 over-expression. Error bars indicated s.d. and were based on five independent experiments. * indicated p < 0.1. ** indicated p < 0.01. *** indicated p < 0.001. NS indicated no significant changes. All p-values were based on two-tailed t-test