Fig. 4.

AFF-1 promotes lumen elongation independently of junction removal. a Confocal Z-projections of L1 stage larvae-expressing apical marker rdy-2(cs233, [rdy-2::gfp]) in green and junction marker ajm-1::mCherry in magenta. d, duct; p, pore. Control animals aff-1( + ) or without heat shock (HS) have a normal lumen morphology. hsp::ZIF-1-induced degradation of AFF-1::ZF1 after heat shock (HS) at the 1.5-fold stage led to a shorter and wider apical domain. b, c Measurements of lumen length and apical domain width, respectively, in animals with normal duct auto-fusion. Error bars = ± SD. Scale bar = 5 μm. **** = p-value < 0.0001, *** = p-value < 0.001, Mann–Whitney test