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. 2018 May 1;8:6817. doi: 10.1038/s41598-018-24922-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) An overlay of the compact and extended conformations POR based on homology models. The calculated Cα-Cα inter-residue distances between N181 and A552 are shown. (B) Average E_Rel dependence on ionic strength. Increasing ionic strength shifts the average conformational equilibrium to low FRET states. Control experiments showing minimal effect of salt concentration on the fluorescence properties of Cy3 (C), Cy5 (D) or the mixture Cy3 and Cy5 (E).