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. 2018 May 1;8:6817. doi: 10.1038/s41598-018-24922-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) A cartoon illustration of the experimental setup (B) Typical trajectory of detected photons recorded from ND reconstituted POR freely diffusing in solution. Coincident bursts correspond to an individual dual labeled POR traversing the diffraction-limited confocal volume. POR labeled with Cy3 or Cy5 only appear as single channel bursts. Inset shows an entire 250 s trace.