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. 2018 May 1;8:6842. doi: 10.1038/s41598-018-25277-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene transfer ability of bPEI and its conjugate. (a) The levels of hIL-12 in OVCAR-3 cells and (b) HepG2 cell following the treatment with polyplexes at C/P ratios of 0.25, 4 and 8. The level of hIL-12 expression was presented as the concentration of the protein (pg/ml) per seeded cells. *p < 0.05, conjugated bPEI derivative compared to unmodified parent polymer at the same C/P ratio (N = 3; error bars represent ± standard deviation).