Table 2. Number of alleles, allele size range, allele frequency, unique allele, gene diversity and polymorphism information content (PIC) of 50 SSR markers across 50 red rice germplasms.
Marker | Chromosome | Motif | Allele(s) | Size Range (bp) |
Size (bp)b | Allele Frequency (%)c | Gene Diversityd | PICe | |||
---|---|---|---|---|---|---|---|---|---|---|---|
# | Position (cM) | No.a | Unique | Rare | |||||||
RM1 | 1 | 29.7 | (GA)26 | 4 | 1 | 0 | 87–112 | 112 | 57.14 | 0.55 | 0.47 |
RM5 | 1 | 94.9 | GA)14 | 2 | 0 | 1 | 110–115 | 115 | 86.00 | 0.24 | 0.21 |
RM35 | 1 | 49.9 | (GA)19 | 3 | 0 | 1 | 200–212 | 212 | 90.00 | 0.18 | 0.18 |
RM312 | 1 | 71.6 | (ATTT)4(GT)9 | 2 | 0 | 0 | 98–104 | 98 | 90.00 | 0.18 | 0.16 |
RM145 | 2 | 49.8 | - | 2 | 0 | 0 | 165–172 | 165 | 94.00 | 0.11 | 0.11 |
RM262 | 2 | 103.3 | (CT)16 | 4 | 0 | 1 | 127–152 | 152 | 82.00 | 0.32 | 0.30 |
RM16 | 3 | 131.5 | (TCG)5(GA)16 | 3 | 2 | 0 | 170–202 | 170 | 96.00 | 0.08 | 0.08 |
RM282 | 3 | 100.6 | (GA)15 | 2 | 1 | 0 | 126–135 | 135 | 98.00 | 0.05min | 0.04min |
RM489 | 3 | 29.2 | (ATA)8 | 3 | 0 | 2 | 242–312 | 242 | 92.00 | 0.15 | 0.15 |
RM554 | 3 | 106.0 | (GA)14 | 2 | 0 | 0 | 251–259 | 259 | 58.00 | 0.49 | 0.37 |
RM518 | 4 | 25.5 | (TC)15 | 3 | 0 | 0 | 164–171 | 171 | 56.00 | 0.59 | 0.52 |
RM537 | 4 | 8.5 | (CCG)9 | 2 | 0 | 1 | 215–221 | 221 | 96.00 | 0.08 | 0.07 |
RM567 | 4 | 153.6 | (GA)21 | 2 | 0 | 0 | 280–289 | 280 | 90.00 | 0.18 | 0.16 |
RM5473 | 4 | 107.35 | (TC)20 | 4 | 0 | 0 | 125–145 | 125 | 60.87 | 0.58 | 0.53 |
RM39 | 5 | 87.7 | (CT)17CCA(TC)3 | 2 | 0 | 0 | 110–115 | 115 | 96.00 | 0.08 | 0.07 |
RM178 | 5 | 118.8 | (GA)5(AG)8 | 2 | 0 | 0 | 117–123 | 117 | 81.63 | 0.30 | 0.25 |
RM334 | 5 | 141.8 | (CTT)20 | 3 | 0 | 0 | 178–190 | 190 | 54.17 | 0.59 | 0.52 |
RM413 | 5 | 26.7 | (AG)11 | 4 | 1 | 0 | 67–105 | 67min | 79.59 | 0.35 | 0.33 |
RM507 | 5 | - | (AAGA)7 | 2 | 0 | 0 | 244–253 | 253 | 86.00 | 0.24 | 0.21 |
RM510 | 6 | 20.8 | (GA)15 | 2 | 0 | 0 | 213–223 | 223 | 94.00 | 0.11 | 0.11 |
RM527 | 6 | 61.2 | (GA)17 | 2 | 0 | 0 | 212–220 | 220 | 78.00 | 0.34 | 0.28 |
RM3330 | 6 | 61.6 | (CT)15 | 3 | 0 | 2 | 136–146 | 146 | 92.00 | 0.15 | 0.15 |
RM70 | 7 | 64.66 | (ATT)33 | 4 | 0 | 0 | 185–211 | 185 | 58.00 | 0.59 | 0.54 |
RM214 | 7 | 34.7 | (CT)14 | 4 | 0 | 0 | 116–144 | 116 | 50.00 | 0.66 | 0.60 |
RM320 | 7 | 62.5 | (AT)11GTAT(GT)13 | 6 | 0 | 2 | 178–212 | 178 | 50.00 | 0.68 | 0.64 |
RM542 | 7 | 34.7 | (CT)22 | 4 | 2 | 0 | 94–122 | 122 | 56.00 | 0.53 | 0.42 |
RM478 | 7 | 93.8 | (AG)12 | 2 | 0 | 0 | 204–210 | 204 | 92.00 | 0.15 | 0.14 |
RM500 | 7 | 36.1 | (AAG)9 | 2 | 0 | 0 | 251–257 | 257 | 60.00 | 0.48 | 0.36 |
RM560 | 7 | 54.2 | (CT)12 | 2 | 1 | 0 | 130–150 | 150 | 98.00max | 0.05min | 0.04min |
RM342 | 8 | 78.4 | (CAT)12 | 3 | 1 | 0 | 132–152 | 152 | 82.00 | 0.30 | 0.27 |
RM447 | 8 | 124.6 | (CTT)8 | 5 | 0 | 1 | 105–139 | 139 | 56.00 | 0.62 | 0.58 |
RM215 | 9 | 99.4 | (CT)16 | 3 | 2 | 0 | 144–164 | 144 | 96.00 | 0.08 | 0.08 |
RM105 | 9 | 32.1 | (CCT)6 | 3 | 1 | 0 | 133–143 | 133 | 86.00 | 0.25 | 0.22 |
RM205 | 9 | 114.7 | (CT)25 | 4 | 1 | 0 | 111–146 | 111 | 68.00 | 0.49 | 0.44 |
RM316 | 9 | 1.8 | (GT)8(TG)9(TTTG)4(TG)4 | 2 | 0 | 0 | 192–199 | 192 | 90.00 | 0.18 | 0.16 |
RM464 | 9 | 3.3 | (AT)21 | 2 | 1 | 0 | 142–150 | 150 | 98.00 | 0.05 | 0.04 |
RM285 | 9 | 1.8 | (GA)12 | 2 | 0 | 0 | 142–150 | 150 | 87.50 | 0.22 | 0.19 |
RM304 | 10 | 73.0 | (GT)2(AT)10(GT)33 | 7 | 2 | 1 | 103–158 | 158 | 30.77min | 0.78max | 0.75max |
RM474 | 10 | - | (AT)13 | 4 | 0 | 0 | 215–248 | 103 | 60.00 | 0.58 | 0.53 |
RM496 | 10 | 113.0 | (TC)14 | 4 | 0 | 0 | 246–285 | 285max | 82.00 | 0.32 | 0.30 |
RM591 | 10 | 118.3 | (AC)10 | 5 | 0 | 2 | 231–272 | 272 | 64.00 | 0.54 | 0.51 |
RM21 | 11 | 85.7 | (GA)18 | 5 | 2 | 0 | 135–160 | 116 | 88.00 | 0.22 | 0.21 |
RM206 | 11 | 102.9 | (CT)21 | 5 | 2 | 0 | 134–172 | 134 | 78.00 | 0.37 | 0.35 |
RM224 | 11 | 120.1 | (AAG)8(AG)13 | 4 | 0 | 0 | 129–145 | 145 | 64.00 | 0.54 | 0.50 |
RM287 | 11 | 68.6 | (GA)21 | 4 | 1 | 1 | 98–115 | 115 | 60.87 | 0.52 | 0.44 |
RM536 | 11 | 55.1 | (CT)16 | 3 | 0 | 0 | 221–237 | 237 | 79.17 | 0.35 | 0.32 |
RM20 | 12 | - | (ATT)14 | 3 | 0 | 0 | 152–163 | 152 | 60.00 | 0.55 | 0.48 |
RM1337 | 12 | - | (AG)21 | 4 | 0 | 1 | 164–185 | 185 | 56.00 | 0.60 | 0.54 |
RM5364 | 12 | - | TC)13 | 4 | 0 | 0 | 127–156 | 156 | 62.50 | 0.54 | 0.48 |
RM7108 | 12 | - | (AGAT)8 | 4 | 1 | 2 | 169–191 | 169 | 56.00 | 0.54 | 0.45 |
aTotal (162), Min (2), Max (7), Avg. (3.24)
bAvg. (167.96)
cTotal (3776.21), Avg. (75.52)
dTotal (17.67), Avg. (0.35)
eTotal (15.87), Avg. (0.32)
Note: Major allele is described as the allele with the highest frequency. Rare alleles are described as alleles with a frequency less than 5%.