Table 1. Primers for q-PCR.
Gene | Sequence | Product Size (bp) | Tm(°C) | Gene bank accession No. |
---|---|---|---|---|
β-actin | F (5'- 3') AAGGACCTCTACG CCAACACG | 255 | 60 | AY141970 |
R (5'- 3') GAAGCATTTGCGG TGGACGAT | ||||
H2A.2 | F (5'- 3') GAGGAGCTGAACAAGCTGTTG | 144 | 60 | BF076713 |
R (5'- 3') TTGTGGTGGCTCTCAGTCTTC | ||||
Bip | F (5'- 3') GCTATTGCTTATGGCCTGGA | 167 | 60 | NM_001075148.1 |
R (5'- 3') CGCTGGTCAAAGTCTTCTCC | ||||
Chop | F (5'-3') GTCACTGCCTTTCTCCTTCG | 218 | 60 | NM_001078163.1 |
R (5'- 3') GGGAGGTGTGTGTGACCTCT | ||||
Ire1 | F (5'- 3') GCTATTGCTTATGGCCTGGA | 170 | 60 | XM_001789477.1 |
R (5'- 3') CTCCATGGCGATCATCTTCT | ||||
Bax | F (5'- 3') TTTGCTTCAGGGTTTCATCC | 246 | 60 | NM_173894.1 |
R (5'- 3') CAGTTGAAGTTGCCGTCAGA | ||||
Bcl-2 | F (5'- 3') ATGTGTGTGGAGAGCGTCAA | 137 | 60 | NM_001166486.1 |
R (5'- 3') TACAGCTCCACAAAGGCGTC | ||||
Hdac1 | F (5'-3') ATCGGTTAGGTTGCTTCAATCTG | 168 | 60 | BT030718.1 |
R (5'- 3') GTTGTATGGAAGCTCATTAGGGA | ||||
Dnmt1 | F (5'- 3') CTCAGAAGGGAGATGTGGAG | 138 | 60 | NM_182651.2 |
R (5'- 3') AGTAGTCACAGTAGCTGAGGA |
F: forward primer; R: reverse primer.