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. 2018 May 2;13(5):e0196504. doi: 10.1371/journal.pone.0196504

Fig 2. Design and testing of a targeting construct for the mouse Gimap6 gene.

Fig 2

Panel A shows the targeting construct in which exons 2 and 3 are flanked by loxP sites, both before and after flpE recombination to remove the selectable neomycin resistance cassette (neo). In addition, DNA fragments encoding thymidine kinase (TK) and diphtheria toxin A chain (DTA) were included in the targeting construct to increase the targeting efficiency in embryonic stem cells. The lower part of panel A shows the integrated DNA fragment after ablation of exons 2 and 3 by Cre-mediated recombination. Panel B shows a Western blot of GIMAP6 (using rat anti-mouse antibody MAC436) and actin on total lymphocytes isolated from thymus or spleen of GIMAP6fl/fl mice, in which the Gimap6 gene is intact, and in GIMAP6fl/flCD2Cre mice, in which Gimap6 should be deleted in the B and T cell populations.