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. 2018 May 2;13(5):e0196854. doi: 10.1371/journal.pone.0196854

Fig 1. SGPL1 expression analysis.

Fig 1

A: RT-PCR with SGPL1 specific primers revealed no significantly altered SGPL1 expression on transcript level for main coding SGPL1 transcript variant 1 (Tv1) as well for the sum of all NCBI annotated SGPL1 transcript variants (Tv all) in comparison with the GAPDH control. B: SGPL1 protein content in the 5 epithelial breast cell lines (20 μg soluble protein) parallel to 10 ng recombinant SGPL1 protein was visualized by western blotting. Non-malignant breast epithelial cell lines MCF-10A and MCF-12A showed strongly elevated SGPL1 protein expression levels. Loading controls were verified by labeling ß-actin and GAPDH proteins as well as by stain-free technology. Uncropped immune blots are shown in S1 Fig.