| Parameters | 488 laser | 561 laser |
| objective | Zeiss W N-Achroplan 10x/0.3 W (DIC) M27 | |
| power | 2% | 2% |
| Pixel dwell | 1.61 | 1.61 |
| Average | 0 | 0 |
| Zoom | 1 | 1 |
| Master gain | 820 | 814 |
| Digital gain | 1 | 0.51 |
| Digital offset | -4.5 | -9.24 |
| Pinhole | 51 | 57 |
| Tunable Filter | 496-553 | 570-618 |
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