| Parameters | 488 laser | 561 laser |
| objective | Plan-Apochromat 63x/1.40 Oil DIC M27 | |
| power | 2% | 2% |
| Pixel dwell | 0.6 | 0.6 |
| Average | 2 (Line) | 2 (Line) |
| Zoom | 2.3 | 2.3 |
| Master gain | 449 | 390 |
| Digital gain | 1 | 1.23 |
| Digital offset | 0 | 0 |
| Pinhole | 136 | 136 |
| Tunable Filter | 493-550 | 566-703 |
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