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Evaluation of anti-rAPC1 serum from mouse. Samples (in micrograms) of W1 pollen protein extract, purified rAPC1, and lysates of E. coli strains expressing GST-rBPC1 fusion protein or GST alone were probed with mouse anti-rAPC1 serum followed by goat-anti-mouse secondary antibody. Mouse preimmune serum or secondary antibody did not detect any proteins (data not shown). Positions of molecular-mass markers (in kilodaltons) are indicated.
