Table I.
Effect of calcium and ionic strength on rBPC1 elution during gel filtration
| Buffer
|
rBPC1 Elution
|
|||||
|---|---|---|---|---|---|---|
| CaCl2 | EGTA | NaCl | Elution Volumea | MWb | MW/Mrc | MW EGTA/MW CaCl2 |
| mm | mL | kD | ||||
| + | − | 0 | 30.3 | 16.8 | 1.6 | — |
| − | + | 0 | 28.5 | 22.3 | 2.1 | 1.3 |
| + | − | 60 | 30.8 | 15.6 | 1.5 | — |
| − | + | 60 | 29 | 20.6 | 1.9 | 1.3 |
| + | − | 200 | 31.3 | 14.4 | 1.4 | — |
| − | + | 200 | 29.5 | 19.2 | 1.8 | 1.3 |
a
Elution volume for peak containing rBPC1 from 1- × 60-cm column of Sephacryl S-100 with flow rate of 0.25 mL/min.
b
Estimated molecular mass (MW) based on calibration with standard proteins (see Methods).
c
Actual Mr of rBPC1 is 10.6.