Skip to main content
. 2018 Mar 14;(133):56866. doi: 10.3791/56866
Surface:
Technique Paraffin Oleamide Petrolatum MP paraffin Permanox Clean glass
phase contrast √*
brightfield √*
DIC N/T x
epifluorescence √*
confocal √*
TIRF N/T x x
PALM/dSTORM/GSDIM N/T x x
SIM N/T N/T N/T N/T N/T N/T
STED N/T N/T N/T N/T
LLSM N/T N/T N/T
Fusion Index intermediate intermediate intermediate high intermediate low
Fusion location random random random defined random random
MP-micropattern
DIC - differential interference contrast
TIRF - total internal reflection fluorescence
PALM - photoactivated localization microscopy
dSTORM - direct stochastic optical reconstruction microscopy
GSDIM - ground state depletion followed by individual molecule return
SIM - structured illumination
STED - stimulated emission depletion
LLSM - lattice light sheet microscopy
√ - compatible
x - incompatible
√* - possible only with LWD, low magnification, or immersion objectives
N/T - not tested