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Surface: |
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Technique |
Paraffin |
Oleamide |
Petrolatum |
MP paraffin |
Permanox |
Clean glass |
phase contrast |
√ |
√ |
√ |
√ |
√* |
√ |
brightfield |
√ |
√ |
√ |
√ |
√* |
√ |
DIC |
√ |
√ |
N/T |
√ |
x |
√ |
epifluorescence |
√ |
√ |
√ |
√ |
√* |
√ |
confocal |
√ |
√ |
√ |
√ |
√* |
√ |
TIRF |
√ |
√ |
N/T |
x |
x |
√ |
PALM/dSTORM/GSDIM |
√ |
√ |
N/T |
x |
x |
√ |
SIM |
N/T |
N/T |
N/T |
N/T |
N/T |
N/T |
STED |
N/T |
√ |
N/T |
N/T |
N/T |
√ |
LLSM |
√ |
N/T |
N/T |
√ |
N/T |
√ |
Fusion Index |
intermediate |
intermediate |
intermediate |
high |
intermediate |
low |
Fusion location |
random |
random |
random |
defined |
random |
random |
MP-micropattern |
DIC - differential interference contrast |
TIRF - total internal reflection fluorescence |
PALM - photoactivated localization microscopy |
dSTORM - direct stochastic optical reconstruction microscopy |
GSDIM - ground state depletion followed by individual molecule return |
SIM - structured illumination |
STED - stimulated emission depletion |
LLSM - lattice light sheet microscopy |
√ - compatible |
x - incompatible |
√* - possible only with LWD, low magnification, or immersion objectives |
N/T - not tested |