Cell marker | Fluorochrome | Clone | Concentration | Amount of antibody per sample |
(in 100 µl of buffer) | ||||
CD45 | vioblue450 | HI30 | 1.0 µg/5µl | 0.4 µg |
CD11b | PE | ICRF44 | 1.0 µg/5µl | 0.4 µg |
CD11c | PerCp/Cy5.5 | Bu15 | 200 µg/ml | 0.4 µg |
CCR5 | PE-Cy7 | 2D7 | 0.25 µg/5µl | 0.1 µg |
CCR7 | PE-Cy7 | REA108 | 99 µg/ml | 0.2 µg |
HLA-DR | BV-570 | L243 | 100 µg/ml | 0.2 µg |
HLA-DR | FITC | AC122 | 82.5 µg/ml | 0.16 µg |
CD3 | APC | UCHT1 | 16 µg/ml | 0.03 µg |
CD3 | viogreen | REA614 | 137 µg/ml | 0.27 µg |
CD163 | APC | GHI/61 | 80 µg/ml | 0.16 µg |
CD207 | APC | 1OE2 | 200 µg/ml | 0.4 µg |
CD1a | FITC | HI149 | 0.5 µg/5µl | 0.2 µg |
CD1a | AF-700 | HI149 | 0.5 mg/ml | 1.0 µg |
CD103 | PE-Cy7 | B-Ly7 | 0.25 µg/5µl | 0.1 µg |
CD83 | PE | HB15e | 0.25 µg/5µl | 0.1 µg |
CD14 | APC-Fire | 63D3 | 400 µg/ml | 0.8 µg |
CD209 | FITC, PE, APC | 120507 | 1.25 µg/0.25ml | 0.01 µg |
Zombie yellow viability dye | ||||
7AAD | 0.1mg/ml | 0.2ug |