Table 2. Small-angle X-ray scattering (SAXS) data.
| Data-collection parameters | |||||
|---|---|---|---|---|---|
| Instrument: | ESRF BM29 | ||||
| Wavelength (Å) | 0.99 | ||||
| q-range (Å−1) | 0.0032–0.49 | ||||
| Sample-to-detector distance | 2.867 m | ||||
| Exposure time (sec/frame) | 1 | ||||
| Temperature (K) | 283 | ||||
| Detector | Pilatus 1M (Dectris) | ||||
| Flux (photons/s) | 1 × 1012 | ||||
| Beam size (µm2) | 172 × 172 | ||||
| Structural parameters | IFT80 | IFT80(BP1-BP2) | IFT80 | ||
| Type of experiment | SEC SAXS | SEC SAXS | Concentration series | Concentration series | |
| Concentration used (mg/mL) | 2 | 2 | 0.47–7.5 | 0.5–8.0 | |
| NaCl concentration (mM) | 150 | 500 | 150 | 150 | |
| From* | Rg (Å) | 38.7 | 34.7 | 26.2 | 41.4–55.7 |
| p(r)* | Dmax (Å) | 122 | 114 | 86.1 | 140.4–194.4 |
| Porod volume Vp x103 (Å3) | 210.8 | 119.8 | 94.3 | 216–347 | |
| Molecular mass (kDa) from Vp | 131.8 | 74.8 | 58.9 | 126–202 | |
| Molecular mass (kDa) from sequence | 170 (dimer) | 85 (monomer) | 65 | 85 | |
| Modeling | |||||
| Dammif | NSD† | 0.597 | 0.795 | ||
*distance distribution function.
†normalized spatial discrepancy between the 10 calculated models.