Figure 4. CDR3s of TcR Alpha chain are diverse.
Amino acid (aa) alignment of TcR αV1 thymocyte clones illustrating diversity of the third complementarity-determining region (CDR3). All clones contain identical variable (V) region sequence (aa 1–61). We grouped clones by shared, identical joining (J) regions (purple boxes) and highlight the differences in the V-J join (CDR3 region) in red boxes.
Figure 4—source data 1. CDR3 regions diversified by exonuclease activity and addition of N and P nucleotides.
Alignment of nucleotides belonging to the join between variable (V) and joining (J) segments within TcRα thymocyte clones. We determined the putative ends of each V segment and putative beginning of each J segment by comparing alignments between different sharks, assuming that identical nucleotides between sharks were germline. The last number of each sequence name indicates the number of clones containing that nucleotide sequence between the V and J segments.
elife-28477-fig4-data1.docx (19.7KB, docx)
DOI: 10.7554/eLife.28477.006