Table 7. List of forward (F) and reverse (R) primers used to generate T cell receptor (TcR) sequences and expression data.

[AID: Activation induced cytidine deaminase; B2M: beta-2 microglobulin; α: alpha; β: beta; γ: gamma; δ: delta; V: variable region; C: constant region].

Primer	F/R	ID	Location	Nucleotide sequence (5' to 3')	Amino acid	Tm
TcR αV1	F	MFC370	leader region of αV1	ATG TTG CCT GAA GCT C	MLPEA	55
	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR αV4	F	MFC122	beginning of αV4	GTC TCC TCA GTT GTT CGT AC	VSSVVR	58
	R	MFC123	end of αV4	CAG TAA TAC ACA GCA GCG TC	DAAVYY	58
	F	MFC374	leader region of αV4	TGG ATT GTG TGG GCA GTA	WIVWAV	54
	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR αV5	F	MFC124	beginning of αV5	CTC AGG AAG GAG AGA TTA TCA C	QEGEII	60
	R	MFC125	end of αV5	CAA TGA TAC ACG GCG GAG TC	DSAVYH	60
	F	MFC124	beginning of αV5	CTC AGG AAG GAG AGA TTA TCA C	QEGEII	60
	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR αV7	F	MFC376	end of leader αV7	AGC GAT GGA GTT TCT GTG ATT	SDGVSVI	58
	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR αV10	F	MFC378	leader region of αV10	CTA TTT CTT CAC TAC CGC AG	YFFTTA	56
	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR α 5'	F	GeneRacer 5' Nested	homologous to RNA oligo	GGA CAC TGA CAT GGA CTG AAG GAG TA	--	78
TcR α 3'	R	MFC191	alpha C region	CAT TGG TGG ATA GCA AGC CCT TCG AT	SKGLLSTN	76
TcR βV1	F	MFC126	beginning of bV1	CTC CGT ACA TCG TCT CTA TTG	PYIVSI	60
	R	MFC127	end of βV1	CAC GCA CAG AAA TAG ACA GC	AVYFCA	58
TcR βV2	F	MFC128	beginning of βV2	CTA CGT GGA GCA GTC TCC ATC	YVEQSP	63
	R	MFC129	end of βV2	GCA CGC ACA ATA ATA GAC AGC C	AVYYCAC	62
TcR βV3	F	MFC130	beginning of βV3	CTA CGT GGA ACA GTC TCC TTC	YVEQSP	61
	R	MFC131	end of βV3	CAC GCG CAG AAA TAG ACA G	VYFCA	57
TcR βV5	F	MFCb50	beginning of βV5	GTT CGG TGC TCT TTC TCT GC	MFGALSLH	60
	R	MFCb54	end of βV5	GAC TGC AGT ATC AGT CGG CAC C	LVPTDTAV	66
TcR γV1	F	MFCg56	beginning of γV1	GTC GCT GTA TTA CTG GCT CAT TG	MSLYYWL	63
	R	MFCg59	end of γV1	GAG CGC ACA GTA ATA GGT GGC AG	TATYYCAL	67
TcR γV3	F	MFCg58	beginning of γV3	GAA GGG TCA CGT CCT TGC G	MKGHVLA	62
	R	MFCg61	end of γV3	GAT CCC AGA GTC ATC CTC	EDDSGI	56
	F	MFC170	beginning of γV3	CAA TAA CCA GAG CAC CGG G	ITRAP	56
	R	MFC171	end of γV3	AGA TCC CAG AGT CGT CCT C	EDDSGI	56
TcR δV3	F	MFCd62	beginning of δV3	GAT TCC CCG TCC CTG GTG TC	DSPSLVS	65
	R	MFCd66	end of δV3	CAG TGC ACA GTG ATA CAC AGC	AVYHCAL	61
TcR δV5	F	MFCd63	beginning of δV5	GCA GCT ACT CAG TAT CTG G	MQLLSIW	57
	R	MFCd67	end of δV5	GAA AGC ACA GTA ATA CAG AG	ALYYCAF	54
TcR δV7	F	MFC172	beginning of δV7	CTG TCA CTC AGT TAT TCT CCT C	VTQLFS	60
	R	MFC173	end of δV7	GCA GCC CAG TTA TAG TCA AAC	LTITGL	60
TcR δV12	F	MFC174	beginning of δV12	CAG AGC CCA CCT CAG TTA C	QSPPQ;	60
	R	MFC175	end of δV12	GAG CGC AGT AAT AGA TGG C	AIYYCA	57
TcR δV16	R	MFC176	end of δV16	GCA GCT CCG AGA TAG ACA AC	LSISEL	60
	F	MFC177	beginning of δV16	GAG TCC TGG CTC ACG CAA TC	ESWLTQ	63
TcR δV17	F	MFC178	beginning of δV17	CAG TCT TGG TCA GAA ATA ACC	QSWSEIT	57
	R	MFC179	end of δV17	CAA CTG AAG ATA AGT GAT CG	ITYLQL	54
AID	F	MFC342	beginning of AID exon 1	AGG CAC GAG ACC TAC ATG TTG	RHETYML	61
	R	MFC347	end of AID exon 2	TGA ACC AGG TGA GGC GGT A	YRLTWF	60
B2M	F	MFC211	first cysteine	AAC GTG TTG CTC TGT CAT GC	NVLLCHA	58
	R	MFC212	before second cysteine	GGG GTG AAC TCC ACA TAA CG	RYVEFTP	60