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Detection of APRX mRNA by in situ hybridization in hypocotyl longitudinal sections taken 1 cm beneath the hook (A–D) or at the hook level (E). A, General view of a section hybridized with the antisense probe. B and C, Detailed views showing differentiating tracheary elements. D, Control section hybridized with the sense probe. Arrows in C indicate the accumulation of transcripts in differentiating xylem elements. Scale bars are in μm.
