Table II.
Initial rates of V-ATPase H+ transport and Na+/H+ exchange activity in tonoplast vesicles isolated from leaves of control and 200 mM NaCl-treated adult M. crystallinum plants treated during a 3-week period, as indicated
| Duration | V-ATPase H+ Transport
|
Na+/H+ Antiport
|
||
|---|---|---|---|---|
| Control | NaCl | Control | NaCl | |
| week | % F mg−1 protein min−1 | |||
| 1 | 668 ± 65 | 904 ± 46 | 164 ± 15 | 215 ± 11 |
| 2 | 693 ± 72 | 1166 ± 55 | 161 ± 12 | 224 ± 15 |
| 3 | 844 ± 40 | 1614 ± 132 | 177 ± 17 | 239 ± 27 |
Plants were 6 weeks old at the beginning of the experiment. Quinacrine fluorescence assays were performed as described in Methods. Values are means ± sd (n = 3 independent experiments).