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. 2018 May 2;8:6937. doi: 10.1038/s41598-018-25204-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of exon 3 inclusion of fl-WT minigene increased TREM2 protein expression. (A) Structure of the 5′ end of unmodified U1 snRNA (cntl) and modified U1 construct based on U1mut3 but matches wild-type (WT) exon 3. (B) Splicing assay of WT(ex2-4) minigene transfected with unmodified U1 snRNA or the U1-ex3 construct in HEK cells. (C) U1-ex3 increased TREM2 protein expression from the fl-WT minigene in HEK cells. Western blot analysis of TREM2 expression (left panel). Bar chart shows the quantification of the western blot results (mean ± SE, n = 4). *P = 0.00013 (-vs. ex3) and P = 0.0007 (cntl vs. ex3) in Tukey’s multiple comparison test. Original gel images and western blot data are shown in Supplementary Fig. S8.