Fig. 4.

Isogenic lines confirm association between plasmepsin II CNV and AUC. a Subclones of KH001_053, identical by a 24-SNP barcode (all clones) and WGS (3 sequenced clones), had variable plasmepsin II CNV levels but only a single pfmdr1 copy. b Isolates with > 90% relatedness vary in plasmepsin II and pfmdr1 CNVs. Plasmepsin II CNV levels correlated with AUC values, confirming that this locus has a role in conferring PPQ resistance. All drug assays were done in triplicate and repeated two times, shown are the means with s.d. CNVs are shown as mean+ s.d. from three biological replicates done in quadruplicate by qPCR a or as average read depth of whole-genome sequencing+ s.d. in b