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. 2018 May 2;8:6934. doi: 10.1038/s41598-018-25121-4

Figure 10.

Figure 10

Relative activity of the upstream regulatory region of T1R1 gene. (A) Schematic representation of the upstream regulatory region of T1R1 gene. A series of plasmids containing the upstream sequences of grass carp T1R1 of different lengths (PGL6-T1R1-P, PGL6-T1R1-CPG1, PGL6-T1R1-CPG3) fused in the frame to the luciferase were co-transfected with pRL-SV40 into HEK293T cells. (B) Relative activity of the upstream regulatory region of T1R1 gene with deletions. Values were normalized to the control plasmid pGL6-Basic and represented as the ratio between firefly and Renilla luciferase activities. The results are expressed as the mean ± SEM arbitrary units of three independent experiments (P < 0.05).