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. 2018 May 2;8:6934. doi: 10.1038/s41598-018-25121-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Responses of HEK293T cells co-expressing grass carp T1R1 and T1R3 to tastant stimuli. (A) Representative radiometric images of fluo-4AM-loaded HEK293T cells during the application of 100 mM L-Arg, L-Lys and L-glu. Top and bottom panels show the cell images that were obtained 0 s and 1 min after stimulation, respectively. (B) The quantification of responses of grass carp T1R1- and T1R3-transfected cells. Amino acids were used at 100 mM. (C) Dose-dependent responses of grass carp T1R1 and T1R3 to L-Arg. Responses were normalized to the mean response before tastant stimuli.