Table 7.

Nucleotide sequences of the primers.

Primers	Sequences (5′-3′)
Primers for cloning of T1R1 and T1R3
CLgc T1R1 F	ATGTTGGTCTGGTGTGTGTTTCTCTC
CLgc T1R1 R	TCACTGCTTGGACATGGTGTACATT
CLgc T1R3 F	ATGGCTAAGGAGTGGACGCTT
CLgc T1R3 R	CTAGCTTTCTTCAGGTGGTGTTGG
Primers for construction of expression vector
EXgc T1R1 F	TAGTCCAGTGTGGTGGAATTCATGTTGGTCTGGTGTGTGTTTCTCTC
EXgc T1R1 R	GGTTTAAACGGGCCCTCTAGATCACTGCTTGGACATGGTGTACATT
EXgc T1R3 F	TAGTCCAGTGTGGTGGAATTCATGGCTAAGGAGTGGACGCTT
EXgc T1R3 R	GGTTTAAACGGGCCCTCTAGA CTAGCTTTCTTCAGGTGGTGTTGG
Primers for genomic DNA amplicon
CPG1 T1R1 F	TGTTTAAGTGCTATATCAGGTCCC
CPG1 T1R1 R	CAAAGGTCTTACGGCTGTGGAAT
CPG2 T1R1 F	GCCGCTCAGATATTGCTCCC
CPG2 T1R1 R	CATTTCTGTCAAACCCAACCTCTG
CPG3 T1R1 F	ACAGAGGTTGGGTTTGACAGAA
CPG3 T1R1 R	GTGTGAGCAAAAACACGCAGTT
Primers for BSP amplicon
BSP1 T1R1 F	AGAAAATATGAAAAAGGATAATTT
BSP1 T1R1 R	ACTTTACCTCGCTTACGAAAAC
BSP2 T1R1 F	GAGAATATTTTTGGTGCGTTAAAA
BSP2 T1R1 R	ATATTATAAAACGACGAAAATATT
BSP3 T1R1 F	AGGTATATGTAGTCGATGAGTTGA
BSP3 T1R1 R	CCCTATTTAAATATAAACAAAAACAC
Primers for cloning of the upstream regulatory region of T1R1 gene
gc T1R1-P F	GGTACCGCTTGGGTGTCGAGGTTGTG
gc T1R1-P R	CTCGAGGTTACTTCCTCTGATATGAGCTCTTC
gc T1R1-CPG1−A F	GGTACCGCTTGGGTGTCGAGGTTGTG
gc T1R1-CPG1−A R	CTTCAAAAATACTTACATTTAAGGCTTGCCAAAACAAAGTT
gc T1R1-CPG1−B F	ACTTTGTTTTGGCAAGCCTTAAATGTAAGTATTTTTGAAGGGTTA
gc T1R1-CPG1−B R	CTCGAGGTTACTTCCTCTGATATGAGCTCTTC
gc T1R1-CPG3−A F	GGTACCGCTTGGGTGTCGAGGTTGTG
gc T1R1-CPG3−A R	GTTTTTGTATGACCCTTTAAAGAAGTTACAACTTTTAGAATGCAT
gc T1R1-CPG3−B F	TTCTAAAAGTTGTAACTTCTTTAAAGGGTCATACAAAAACTGCGT
gc T1R1-CPG3−B R	CTCGAGGTTACTTCCTCTGATATGAGCTCTTC
Primers for real-time PCR
RTgc EF1 F	GCTGACTGTGCCGTGCTGAT
RTgc EF1 R	GCTGACTTCCTTGGTGATTTCC
RTgc T1R1 F	CTGCCGCCACCTTCCTCA
RTgc T1R1 R	CTCCGGCCGACTTCACCAC
RTgc T1R3 F	CACAGGATGGTCTCTGAACG
RTgc T1R3 R	ATAGGGACTGAATAGGCGAA