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. 2018 May 2;8:6893. doi: 10.1038/s41598-018-25225-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum Likelihood phylogenetic tree of the partial COI sequence data set of 19 Epimeria species and Gammarus locusta as outgroup. Model of choice: HKY with a log likelihood score of −6124.0843, a discrete gamma distribution (+G, parameter = 1.0064) and a proportion of invariant sites (+I, 46% of sites). Numbers next to internal branches are non-parametric bootstrap values (in %). Only values above 90% are shown. Initial tree(s) for the heuristic search were obtained automatically by applying BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then by selecting the topology with superior log likelihood value. The tree is drawn to scale with branch lengths measured in substitutions per site. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 542 positions in the final dataset.