Fig. 2.

K562 cell apoptosis and survival affected by DDX43. a DDX43-K562 or control cells were cultured for 48 h. Expression of DDX43 inhibited cell apoptosis. b DDX43-K562 or control cells were cultured in serum-free conditions. DDX43 promoted the survival of transfected cells. c Expression of DDX43 in DDX43-Meg-01 or control cells were detected by RQ-PCR. d, e Cell survival and apoptosis were assayed in Meg-01. f Expression of DDX43 in DDX43 shRNA or control vector transfected DDX43-K562 cells was detected by western blot. Lanes 1–3: DDX43-K562 transfected with three shRNAs; Lane 4: DDX43-K562 transfected with a negative control. ShRNA-1 significantly decreased DDX43 expression. g Cell survival was assayed in DDX43 shRNA-1-transfected K562. Error bars indicate SD (n = 3). *P < 0.05; **P < 0.01