Fig. 5.

DDX43 promoted H19 expression by demethylation. a–d Expression of H19 in DDX43-transfected K562, HL60, SHI-1 and DDX43-silencing K562 cells was detected by RQ-PCR. e, f Promoter methylation levels of H19 in control vector or DDX43 transfected cells were detected by bisulfite sequencing. g Expression of H19 was detected by RQ-PCR. h K562 cells transfected with H19 siRNA or a control was used to apoptotic assays in serum-free media for 48 h. Cells were subjected to staining, followed by flow cytometry analysis. i K562 cells transfected with H19 siRNA or a control were subjected to proliferation assays in 10% FBS media and the live cells were counted at 24, 48, and 72 h. *P < 0.05; **P < 0.01. Error bars indicate SD (n = 3)