Fig. 6.

miR-186-mediated DDX43 functions. a Expression of miR-186 in DDX43 or control vector transfected K562 cells was detected by RQ-PCR. b Binding of miR-186 to DDX43 3′ UTR was detected by luciferase assay. c The wild-type (Luc-DDX43) and mutant (Luc-DDX43-Mut) 3′ UTR sequences of DDX43 for luciferase activity assays. d Bioinformatics analysis showed the binding of miR-186 to c-Myc 3′ UTR. e, f Expression of c-Myc in DDX43- or control vector-transfected cells was detected by western blot and RQ-PCR. g K562 cells transfected with miR-186 mimic or control vector were cultured in serum-free media. Cells were harvested and surviving cells were counted. h, i K562 cells transfected with miR-186 mimic or a control were harvested for apoptotic assays in serum-free media after 48 h. Cells were subjected to staining, followed by flow cytometry analysis. j Relative expression level of miR-186 in CP and AP/BC stage of CML patients was presented with scatter plots. The median level of miR-186 in each group was shown with horizontal line. *P < 0.05; **P < 0.01. Error bars indicate SD (n = 3)