Table 2.
Data collection and scattering-derived parameters.
| Data collection parameters | ||
| WT | Q383A | |
| Beamline | ESRF BM29 | |
| Beam geometry (mm2) | 0.7 × 0.7 | |
| Wavelength (Å) | 1.0 | |
| Q range (Å−1) | 0.0025–0.5 | |
| No. of frames | 10 | 20 |
| Exposure time per frame (seconds)1 | 0.5 | 1 |
| Samples concentrations (mg/ml) | 4.1, 1.6, 1.1 | 0.9, 0.43, 0.2 |
| Temperature (°C) | 5 | |
| Structural parameters | ||
| Rg (Å) [from P(r)]1 | 22.6 ± 0.004 | 26.3 ± 0.01 |
| Rg (Å) (from Guinier)1 | 22.4 ± 0.1 | 25.8 ± 0.4 |
| Dmax (Å)2 | 75 ± 8 | 85 ± 9 |
| Porod volume [from P(r)] (103 Å3) | 42.53 | 50.75 |
| NSD3 | 0.77 ± 0.04 | 0.55 ± 0.05 |
| χ2 CRYSOL | 3.8 | 5.2 |
| χ2 EOM | 1.0 | 1.1 |
| Software employed | ||
| Primary data reduction | AUTOMAR | |
| Data processing | PRIMUS | |
| Modelling | DAMMIF, EOM | |
1±S.E.
2±10% (estimated range).
3NSD, normalized shape discrepancy for DAMMIF calculation.