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. 2018 May 2;8:6897. doi: 10.1038/s41598-018-24621-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival tests of early embryos derived from Oryzias celebensis pairs (A) O. woworae pairs (A) O. celebensis females with O. woworae males (B), O. woworae females with O. celebensis males (B) the F₁ hybrid females with O. woworae males (C) and backcross (BC) females with O. woworae males (D). Mating schemes are shown above each graph. Eggs, spawned and fertilised in fresh water, were transferred to fresh water (FW) or sea water (SW) after 1 h (1 to 2 cell stage). Survival ratios 24 h after fertilisation were calculated at least 20 eggs/embryos from five pairs (A–C) or 72 pairs (D). Open and closed squares indicate survival ratios of embryos from each female. Groups with different letters in each graph are significantly (P < 0.05) different. f, female; m, male; cel, O. celebensis; wow, O. woworae.