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. 2018 May 2;8:6917. doi: 10.1038/s41598-018-25087-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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No expression of Ndrg1-CreERT2 in the parathyroid glands. (a) Cre expression study in the parathyroid glands of Ndrg1-CreERT2;tdTomato mice at 3 weeks after tamoxifen treatment. Parathyroid sections were immunostained for parathyroid hormone (PTH, green) and RFP (red), and counterstained for cell nuclei with DAPI (blue). Cre was not expressed in PTH-positive parathyroid cells. (b) αKlotho expression study in the parathyroid glands of Ndrg1-CreERT2;αKlotho^flox/flox mice and αKlotho^flox/flox control mice at 3 weeks after tamoxifen treatment. Parathyroid sections were immunostained for αKlotho (red) and PTH (green), and counterstained for cell nuclei with DAPI (blue). αKlotho was expressed equally in PTH-positive parathyroid cells of both mouse strains. Scale bars: 50 μm.