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. 2018 May 2;8:6947. doi: 10.1038/s41598-018-25170-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations between APD₉₀ and FPD in cardiac myocyte cultures after the L-type Ca²⁺ channel blockade. APD₉₀ (a) and FPD (b) restitution relations before and after the application of 772nmol/L nifedipine (n = 8 for each group of data). APD₉₀ and FPD differed significantly before and after the treatment with nifedipine (p < 0.001 and p < 0.0001 respectively, ordinary two-way ANOVA). (c) The linear relationship between APD₉₀ and FPD (r² = 0.86, slope = 0.93, p < 0.0001). Linear regression analysis for the relationship between (d) APD₈₀ (r² = 0.77, slope = 0.89, p < 0.0001) or (e) APD₇₀ (r² = 0.75, slope = 0.88, p < 0.0001) and FPD. (f) Superimposed raw traces obtained before and after suppression of I_CaL by nifedipine and the concurrent EGMs, all derived from the same electrode on the left. Mean ± SEM; ***p < 0.001; ****p < 0.0001.