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. 2018 May 2;8:6911. doi: 10.1038/s41598-018-25083-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The signal size during repeated patch clamp measurements. (A) Normalized internal and external membrane optical signal size for different trials in response to the 100 mV step. HEK239 cells expressing Aahn were voltage-clamped directly. (B) Normalized internal and external membrane optical signal size in response to the 100 mV step. HEK239 cells expressing Aahn were voltage-clamped indirectly. (C) Normalized internal and external membrane optical signal size in response to 50 mV steps. HEK239 cells expressing Aahn were voltage-clamped indirectly. (D) Normalized internal and external membrane optical signal size in response to the 100 mV step with a −35 mV holding potential. HEK239 cells expressing Aahn were voltage-clamped indirectly. The error bars represent the standard error of the mean (n = 3 cells for each plot).