Table 2.
Open reading frames (ORFs) encoding nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) in NRPS and PKS gene clusters from Streptomyces sp. TP-A0882 (NBRC 110030).
| Gene cluster | Presumed product | ORF (accession)a | Size (aa) | Domain organization |
|---|---|---|---|---|
| nrps-1 | coelibactin | 12–265 (WP_055468803)c | 554 | A(dhb) |
| 12–266 (WP_055468804) | 2,246 | T-C/A/T-C/A(cys)/T | ||
| 12–267 (WP_055468805) | 1,857 | C/A(cys)/MT/T-TE | ||
| nrps-2 | coelichelin | 12–104 (WP_055468733) | 3,644 | A(orn)/T/E-C/A(thr)/T/E-C/A(orn)/T |
| nrps-3 | mCys-Val-…-x-Ser | 13–1 (in BBOK01000009) b | >2,354 | C/A(cys)/MT/T-C/A(val)… |
| 22–1 (in BBOK01000019) b | >2,560 | …E-C/A/T-C/A(ser)/T | ||
| nrps-4 | thiazostatin | 2–333 (WP_055468178) | 1,829 | C/A(cys)/MT/T-TE |
| 2–328 (WP_055468176) | 1,523 | T-C/A(cys)/T | ||
| 2–326 (WP_053639878) | 532 | A(dhb) | ||
| pks/nrps-1 | x-Val-Pro-pk | 10–54 (WP_055469571) | 1,303 | C/A/T-TE |
| 10–53 (WP_063788334) | 3,113 | A(val)/T-C/A(pro)/T-KS/KR/ACP-TE | ||
| t1pks-1 | butyrolactol | 10–11 (WP_055469545)c | 6,065 | AT/ACP-KS/AT(mm)/DH/ER/KR/ACP-KS/AT(m)/KR/ACP-KS/AT(m)/DH/KR/ACP |
| 10–14 (WP_055469666) | 2,083 | KS/AT(m)/DH/ER/KR/ACP | ||
| 10–15 (WP_055469548) | 3,365 | KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP | ||
| 10–16 (WP_055469549) | 3,462 | KS/AT/DH/ER/KR/ACP-KS/KR/ACP | ||
| 10–17 (WP_055469550) | 3,135 | KS/AT(m)/KR/ACP-KS/AT(m)/KR/ACP | ||
| 10–18 (WP_055469551) | 1,169 | KS/DH/KR/ACP | ||
| t1pks-2 | AHBA-diketide | 2–307 (WP_055468168) | 2,191 | CoL(AHBA)/KR/ACP-KS/AT(m)/ACP |
| 2–306 (WP_055468167) | 1,296 | KS/AT(m)/ACP-TE | ||
| t1pks-3 | unknown | 18–62 (WP_063788240) | 128 | ACP |
| 18–61 (WP_055468074) | 2,027 | KS/AT(m)/DH/ER/KR/ACP | ||
| 18–60 (WP_051849763) | 482 | KS | ||
| other t1pks(s) | unknown(s) | 26–1 (WP_055470054) | >1,045 | …AT(m)/DH/KR/ACP |
| 26–2 (WP_055470053) | 1,715 | KS/AT(mm)/KR/ACP | ||
| 26–3 (in BBOK01000023) b | >2,325 | KS/AT(m)/DH/KR/ACP-KS… | ||
| 13–248 (WP_055468920) | >354 | …DH/KR/ACP | ||
| 13–247 (WP_055468919) | 3,111 | KS/AT(m)/DH/KR/ACP-KS/AT(m)/ACP-TE | ||
| 13–232 (WP_055468914) | 5,409 | ACP-KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP | ||
| 13–231 (WP_055468913) | 1,862 | KS/AT(m)/DH/KR/ACP | ||
| t2pks-1 | gray spore pigment | 7–97 (WP_030402764) | 423 | KS |
| 7–98 (WP_053637533) | 422 | KS | ||
| 7–99 (WP_030402766) | 89 | ACP | ||
| t2pks-2 | kinamycin-like | 15–178 (WP_031082067) | 423 | KS |
| 15–179 (WP_031184969) | 407 | KS | ||
| 15–180 (WP_030402549) | 89 | ACP | ||
| t3pks-1 | THN | 4–414 (WP_031081839) | 374 | KS |
| t3pks-2 | phenolic lipid | 7–128 (WP_037824347) | 390 | KS |
| t3pks-3 | unknown | 4–314 (WP_030398736) | 361 | KS |
Abbreviations: A, adenylation; ACP, acyl carrier protein; AHBA, aminohydroxybenzoic acid; AMT, aminotransferase; AT, acyltransferase; C, condensation; CoL, CoA ligase; DH, dehydratase; E, epimerization; ER, enoylreductase; F, formyltransferase; KR, ketoreductase; KS, ketosynthase; m, malonyl-CoA: mCys, methyl-cysteine; mGly, methyl-glycine; mm, methylmalonyl-CoA; MT, methyltransferase; pk, moiety derived from PKS pathway; T, thiolation; TD, termination; TE, thioesterase; THN, tetrahydroxynaphthalene; x, unidentified amino-acid; y, unknown building block because A domain is not present in the module. Predicted substrates of A, AT, and CoL domains are shown in brackets.
aORFs are shown as a combination of scaffold number and ORF number. Incompletely sequenced ORFs are shown in italics, and undetermined domains are shown as “…”.
bBecause the ORFs are not registered in GenBank, accession numbers for the DNA sequences encoding each ORF are instead indicated in brackets.
cEncoded on the complementary strand.