Table 4.
ORFs encoding NRPSs and PKSs in NRPS and PKS gene clusters from S. diastaticus subsp. ardesiacus NBRC 15402T.
| Gene cluster | Presumed product | ORFa | Size (aa) | Domain organization | Closest homolog (accession, origin, % of identity/similarity)b |
|---|---|---|---|---|---|
| nrps-1 | coelibactin | 1–1240c | 542 | A(dhb) | WP_055468803, Streptomyces sp. NBRC 110030, 97/97 |
| 1–1241 | 2,213 | T-C/A/T-C/A(cys)/T |
KOX46963, Streptomyces sp. NRRL F-7442, 99/99 (WP_055468804, Streptomyces sp. NBRC 110030, 99/99) |
||
| 1–1242 | 1,857 | C/A(cys)/MT/T-TE |
WP_053663986, Streptomyces sp. NRRL F-7442, 99/99 (WP_055468805, Streptomyces sp. NBRC 110030, 99/99) |
||
| nrps-2 | coelichelin | 1–1084 | 3,644 | A(orn)/T/E-C/A(thr)/T/E-C/A(orn)/T |
KOX26695, Streptomyces sp. NRRL F-4707, 99/99 (WP_055468733, Streptomyces sp. NBRC 110030, 99/99) |
| nrps-3 | mCys-Val-x-x-Ser | 1–84 | 3,616 | C/A(cys)/MT/T-C/A(val)/T/E-C | WP_051908973, Streptomyces sp. NRRL F-5635, 99/99d |
| 1–85 | 3,241 | A/T/E-C/A/T-C/A(ser)/T | EHN79578, Streptomyces coelicoflavus ZG0656, 93/94d | ||
| nrps-4 | thiazostatin | 10–229 | 1,829 | C/A(cys)/MT/T-TE |
WP_031081050, Streptomyces sp. NRRL S-1831, 99/99 (WP_055468178, Streptomyces sp. NBRC 110030, 99/99) |
| 10–234 | 1,535 | T-C/A(cys)/T |
WP_031184402, Streptomyces sp. NRRL F-5635, 99/98 (WP_055468176, Streptomyces sp. NBRC 110030, 98/98) |
||
| 10–236 | 532 | A(dhb) | WP_053639878, Streptomyces sp. NBRC 110030, 99/99 | ||
| pks/nrps-1 | x-Val-Pro-pk | 5–41 | 1,303 | C/A/T-TE | WP_055469571, Streptomyces sp. NBRC 110030, 99/99 |
| 5–40 | 3,105 | A(val)/T-C/A(pro)/T-KS/KR/ACP-TE | WP_063788334, Streptomyces sp. NBRC 110030, 99/99 | ||
| t1pks-1 | butyrolactol | 43–30c | 6,062 | AT/ACP-KS/AT(mm)/DH/ER/KR/ACP-KS/AT(m)/KR/ACP-KS/AT(m)/DH/KR/ACP | WP_055469545, Streptomyces sp. NBRC 110030, 99/99 |
| 43–33 | >398 | KS… | WP_055469666, Streptomyces sp. NBRC 110030, 98/98 | ||
| 58–1 | >1,655 | …AT/DH/ER/KR/ACP | WP_055469666, Streptomyces sp. NBRC 110030, 98/98 | ||
| 58–2 | >426 | KS… | WP_055469548, Streptomyces sp. NBRC 110030, 99/99 | ||
| 62–1 | >1,601 | …AT(m)/DH/KR/ACP-KS… | WP_055469548, Streptomyces sp. NBRC 110030, 97/98 | ||
| 5–1 | >1,334 | …AT(m)/DH/KR/ACP | WP_055469548, Streptomyces sp. NBRC 110030, 97/98 | ||
| 5-2 | 3,464 | KS/AT/DH/ER/KR/ACP-KS/KR/ACP | WP_055469549, Streptomyces sp. NBRC 110030, 99/99 | ||
| 5-3 | 3,141 | KS/AT(m)/DH/KR/ACP-KS/AT(m)/KR/ACP |
KOT98773, Streptomyces sp. NRRL F-4711, 99/99 (WP_055469550, Streptomyces sp. NBRC 110030, 99/99) |
||
| 5-4 | 1,169 | KS/DH/KR/ACP |
KOT98774, Streptomyces sp. NRRL F-4711, 99/99 (WP_055469551, Streptomyces sp. NBRC 110030, 99/99) |
||
| t1pks-2 | AHBA-diketide | 10–255 | 2,191 | CoL(AHBA)/KR/ACP-KS/AT(m)/ACP |
WP_051908920, Streptomyces sp. NRRL F-5635, 99/99 (WP_055468168, Streptomyces sp. NBRC 110030, 99/99) |
| 10–256 | 1,296 | KS/AT(m)/ACP-TE |
WP_053663292, Streptomyces sp. NRRL F-7442, 99/99 (WP_055468167, Streptomyces sp. NBRC 110030, 99/99) |
||
| t1pks-3 | unknown | 6–621 | 128 | ACP | WP_063788240, Streptomyces sp. NBRC 110030, 99/100 |
| 6–622 | 2,027 | KS/AT(m)/DH/ER/KR/ACP |
KOX28560, Streptomyces sp. NRRL F-4707, 99/99 (WP_055468074, Streptomyces sp. NBRC 110030, 99/100) |
||
| 6–623 | 482 | KS |
WP_051783751, Streptomyces sp. NRRL F-5555, 99/99 (WP_051849763, Streptomyces sp. NBRC 110030, 99/99) |
||
| other t1pks(s) | unknown(s) | 53-3 | >1,507 | …AT(m)/DH/KR/ACP | WP_055470054, Streptomyces sp. NBRC 110030, 99/98 |
| 53-2 | 1,650 | KS/AT(mm)/KR/ACP |
KOX41189, Streptomyces sp. NRRL F-7442, 99/99 (WP_055470053, Streptomyces sp. NBRC 110030, 99/99) |
||
| 53-1 | >2,463 | KS/AT(m)/DH/KR/ACP-KS/AT… | WP_033305239, Streptomyces atroolivaceus, 57/67d | ||
| 77-1 | >762 | …KR/ACP-KS… | AHH99923, Kutzneria albida DSM 43870, 63/74 | ||
| 80-1 | >489 | …KS… | WP_040741646, Nocardia tenerifensis, 70/78 | ||
| 59-1 | >350 | …KR/ACP | WP_055468920, Streptomyces sp. NBRC 110030, 100/100 | ||
| 59-2 | >1,370 | KS/AT(m)/DH… | WP_055468919, Streptomyces sp. NBRC 110030, 99/99 | ||
| 20-1 | >1,605 | …KR/ACP-KS/AT(m)/ACP-TE | WP_055468919, Streptomyces sp. NBRC 110030, 99/99 | ||
| 20-16 | 5,412 | ACP-KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP |
WP_053639270, Streptomyces sp. NRRL F-4707, 99/99 (WP_055468914, Streptomyces sp. NBRC 110030, 99/99) |
||
| 20-17 | 1,862 | KS/AT(m)/DH/KR/ACP |
WP_053639271, Streptomyces sp. NRRL F-4707, 99/99 (WP_055468913, Streptomyces sp. NBRC 110030, 99/99) |
||
| t2pks-1 | gray spore pigment | 2–814 | 423 | KS | WP_030402764, Streptomyces sp. NBRC 110030, 100/100 |
| 2–813 | 422 | KS | WP_053637533, Streptomyces sp. NBRC 110030, 99/100 | ||
| 2–812 | 89 | ACP | WP_030402766, Streptomyces sp. NBRC 110030, 100/100 | ||
| t2pks-2 | kinamycin-like | 15–126 | 423 | KS |
KOX34713, Streptomyces sp. NRRL F-4707, 100/100 (WP_031082067, Streptomyces sp. NBRC 110030, 99/100) |
| 15–127 | 407 | KS |
WP_031184969, Streptomyces sp. NRRL F-5635, 99/100 (WP_055468989, Streptomyces sp. NBRC 110030, 99/99) |
||
| 15–128 | 89 | ACP | WP_030402549, Streptomyces sp. NBRC 110030, 99/100 | ||
| t3pks-1 | THN | 8–149 | 374 | KS | WP_031081839, Streptomyces sp. NBRC 110030, 100/100 |
| t3pks-2 | phenolic lipid | 1–740 | 390 | KS | WP_037824347, Streptomyces sp. NBRC 110030, 99/99 |
| t3pks-3 | unknown | 10–248 | 361 | KS | WP_030398736, Streptomyces sp. NBRC 110030, 100/100 |
Abbreviations are the same as those of Table 2.
aORFs are shown as a combination of scaffold number and ORF number. Incompletely sequenced ORFs are shown in italics, and undetermined domains are shown as “…”.
bParentheses indicate that the closest homolog is not from Streptomyces sp. TP-A0882 (NBRC 110030).
cEncoded on the complementary strand.
dAlthough homologs in Streptomyces sp. TP-A0882 did not appear as high score hits in basic local alignment search tool analyses because they are not registered in GenBank, they are present in scaffolds 13 (BBOK01000009), 22 (BBOK01000019), and 26 (BBOK01000023) of the Streptomyces sp. TP-A0882 genome.