Table 5.
ORFs encoding NRPSs and PKSs in NRPS and PKS gene clusters of S. coelicoflavus NBRC 15399T.
| Gene cluster | Presumed product | ORFa | Size (aa) | Domain organization | Closest homolog (accession, origin, % of identity/similarity)b |
|---|---|---|---|---|---|
| nrps-i | coelibactin | 3–140c | 554 | A(dhb) |
EHN75391, S. coelicoflavus ZG0656, 99/99 (CAC17498, S. coelicolor A3(2), 85/89) |
| 3–141 | 2,250 | T-C/A/T-C/A(cys)/T |
EHN75408, S. coelicoflavus ZG0656, 99/99 (CAC17499, S. coelicolor A3(2), 86/89) |
||
| 3–142 | 1,857 | C/A(cys)/MT/T-TE |
EHN75409, S. coelicoflavus ZG0656, 99/99 (CAC17500, S. coelicolor A3(2), 89/91) |
||
| nrps-i | coelichelin | 6–362 | 3,666 | A(orn)/T/E-C/A(thr)/T/E-C/A(orn)/T |
KPC76200, Streptomyces sp. NRRL WC-3753, 99/99 (EHN78004, S. coelicoflavus ZG0656, 99/98; CAB53322, S. coelicolor A3(2), 86/90) |
| nrps-iii | x-x-Ser-mCys-Val-x-x-Ser | 3–549 | 3,637 | C/A(cys)/MT/T-C/A(val)/T/E-C/A/T/E-C/A/T-C/A(ser)/T | EHN75118, S. coelicoflavus ZG0656, 99/99 |
| 3–550 | 3,271 | A/T/E-C/A/T-C/A(ser)/T | EHN79578, S. coelicoflavus ZG0656, 99/98 | ||
| nrps-iv | Gly-y-Asp-Tyl-Thr-x-Asp-Gly-Pro-Gly-Gly-Ala-mGly | 2–543 | 6,937 | C/A(gly)/T-C/T-C/A(asp)/T/E-C/A(tyl)/T-C/A(thr)/T-C/A/T/E |
KPC71694, Streptomyces sp. NRRL WC-3753, 99/99 (EHN72150, S. coelicoflavus ZG0656, 99/99) |
| 2–544 | 4,213 | C/A(asp)/T-C/A(gly)/T-C/A(pro)/T-C/A(gly)/T |
KPC71705, Streptomyces sp. NRRL WC-3753, 99/99 (EHN72136, S. coelicoflavus ZG0656, 99/99) |
||
| 2–545 | 3,865 | C/A(gly)/T-C/A(ala)/T-C/A(gly)/MT/T-TE |
WP_054100963, Streptomyces sp. NRRL WC-3753, 99/99 (EHN72117, S. coelicoflavus ZG0656, 99/99) |
||
| pks/nrps-i | prodiginine | 3–99 | 1,012 | KS/KS |
KPC87173, Streptomyces sp. NRRL WC-3753, 99/99 (EHN75481, S. coelicoflavus ZG0656, 99/99; CAA16487, S. coelicolor A3(2), 91/94) |
| 3–107c | 407 | KS |
EHN75487, S. coelicoflavus ZG0656, 100/100 (CAA16177, S. coelicolor A3(2), 94/97) |
||
| 3–108c | 81 | ACP |
EHN75488, S. coelicoflavus ZG0656, 100/100 (CAA16178, S. coelicolor A3(2), 96/97) |
||
| 3–110 | 87 | ACP |
EHN77254, S. coelicoflavus ZG0656, 100/100 (CAA16180, S. coelicolor A3(2), 95/95) |
||
| 3–111 | 636 | ACP/AMT |
KPC87185, Streptomyces sp. NRRL WC-3753, 99/98 (EHN75478, S. coelicoflavus ZG0656, 98/98; CAA16181, S. coelicolor A3(2), 88/89) |
||
| 3–112 | 532 | A(cys) |
KPC87186, Streptomyces sp. NRRL WC-3753, 99/99 (EHN75480, S. coelicoflavus ZG0656, 99/99; CAA16182, S. coelicolor A3(2), 93/95) |
||
| 3–113 | 2,306 | CoL(NH2)/T-KS/AT(m)/ACP/AMT |
EHN77210, S. coelicoflavus ZG0656, 99/99 (CAA16183, S. coelicolor A3(2), 87/90) |
||
| 3–115 | 280 | TE |
EHN75475, S. coelicoflavus ZG0656, 99/99 (CAA16185, S. coelicolor A3(2), 96/96) |
||
| pks/nrps-ii | oxazolomycin-like | 7–245c | 842 | ACP-TD | WP_051005867, S. coelicoflavus ZG0656, 98/98 |
| 7–244c | 1,752 | KS/ACP-C/FkbH |
WP_054101954, Streptomyces sp. NRRL WC-3753, 99/98 (WP_051005868, S. coelicoflavus ZG0656, 98/98) |
||
| 7–242 | 2,968 | DH/ACP/ACP/ACP/DH-KS/KR/ACP-KS/ACP |
WP_054101951, Streptomyces sp. NRRL WC-3753, 97/97 (EHN75054, S. coelicoflavus ZG0656, 97/97) |
||
| 7–241 | 3,008 | C/A(ser)/T-C/A/MT/T-C |
KPC72343, Streptomyces sp. NRRL WC-3753, 99/99 (EHN75030, S. coelicoflavus ZG0656, 99/99) |
||
| 7–237 | 4,903 | KS/DH/KR/ACP-KS/DH/KR/ACP-KS/DH/KR/MT/ACP |
KPC72421, Streptomyces sp. NRRL WC-3753, 98/98 (EHN75036, S. coelicoflavus ZG0656, 97/97) |
||
| 7–236 | 1,158 | F/A(gly)/T |
KPC71002, Streptomyces sp. NRRL WC-3753, 99/99 (EHN77489, S. coelicoflavus ZG0656, 99/99) |
||
| 7–234 | 879 | KS/ACP |
KPC71004, Streptomyces sp. NRRL WC-3753, 99/99 (EHN75023, S. coelicoflavus ZG0656, 99/99) |
||
| 7–233 | 6,079 | KS/KR/MT/ACP-C/A(gly)/T-KS/DH/KR/ACP-KS/KR/ACP-KS |
WP_054102642, Streptomyces sp. NRRL WC-3753, 98/98 (EHN78704, S. coelicoflavus ZG0656, 98/98) |
||
| 7–232 | 1,106 | AT/AT(m) | EHN78700, S. coelicoflavus ZG0656, 99/99 | ||
| t2pks-i | gray spore pigment | 11–215 | 423 | KS |
KPC88984, Streptomyces sp. NRRL WC-3753, 100/100 (EHN75824, S. coelicoflavus ZG0656, 99/99; CAB45606, S. coelicolor A3(2), 98/99) |
| 11–214 | 424 | KS |
KPC88985, Streptomyces sp. NRRL WC-3753, 99/99 (EHN75823, S. coelicoflavus ZG0656, 98/98; CAB45607, S. coelicolor A3(2), 98/98) |
||
| 11–213 | 89 | ACP |
EHN75822, S. coelicoflavus ZG0656, 100/100 (CAB45608, S. coelicolor A3(2), 98/98) |
||
| t2pks-ii | unknown | 1–30 | 84 | ACP | EHN79053, S. coelicoflavus ZG0656, 100/100 |
| 1–31 | 422 | KS | EHN79055, S. coelicoflavus ZG0656, 100/100 | ||
| 1–32 | 416 | KS | EHN79056, S. coelicoflavus ZG0656, 99/99 | ||
| t2pks-iii | unknown | 14–63 | 421 | KS | EHN77732, S. coelicoflavus ZG0656, 100/100 |
| 14–62 | 415 | KS |
KPC71304, Streptomyces sp. NRRL WC-3753, 99/99 (EHN77731, S. coelicoflavus ZG0656, 99/99) |
||
| t3pks-i | THN | 5–164 | 374 | KS |
EHN79529, S. coelicoflavus ZG0656, 100/100 (CAC01488, S. coelicolor A3(2), 91/95) |
Abbreviations are the same as those of Table 2.
aORFs are shown as a combination of scaffold number and ORF number.
bIf the homolog in S. coelicoflavus ZG0656 is not the closest and/or Streptomyces coelicolor A3(2) harbors the homolog, it is shown in parentheses.
cEncoded on the complementary strand.