Table 6.
ORFs encoding NRPSs and PKSs in NRPS and PKS gene clusters of S. rubrogriseus NBRC 15455T.
| Gene cluster | Presumed product | ORFa | Size (aa) | Domain organization | Closest homolog (accession, origin, % of identity/similarity)b |
|---|---|---|---|---|---|
| nrps-a | coelibactin | 7–361c | 553 | A(dhb) | CAC17498, S. coelicolor A3(2), 98/98 |
| 7–362 | 2,240 | T-C/A/T-C/A(cys)/T | CAC17499, S. coelicolor A3(2), 96/97 | ||
| 7–363 | 1,842 | C/A(cys)/MT/T-TE | CAC17500, S. coelicolor A3(2), 97/97 | ||
| nrps-b | coelichelin | 4–1115 | 3,649 | A(orn)/T/E-C/A(thr)/T/E-C/A(orn)/T | CAB53322, S. coelicolor A3(2), 95/96 |
| nrps-c | CDA | 13–202 | 7,395 | C/A(ser)/T-C/A(thr)/T-C/A(trp)/T/E-C/A(asp)/T-C/A(asp)/T-C/A(hpg)/T/E | CAB38518, S. coelicolor A3(2), 95/96 |
| 13–203 | 3,658 | C/A(asp)/T-C/A(gly)/T-C/A(asn)/T/E | CAB38517, S. coelicolor A3(2), 96/97 | ||
| 13–204 | 2,429 | C/A/T-C/A(trp)/T-TE | CAD55498, S. coelicolor A3(2), 97/97 | ||
| nrps-d | x-y-Cys | 1–88 | 1,177 | A/T-C/T |
SDT78734, Streptomyces sp. 2114.2, 98/98 (CAA18918, S. coelicolor A3(2), 98/98) |
| 1–89 | 1,413 | C/A(cys)/T-TE | CAA18919, S. coelicolor A3(2), 96/96 | ||
| pks/nrps-a | prodiginine | 3–389 | 932 | KS/KS | CAA16487, S. coelicolor A3(2), 96/96 |
| 3–381c | 407 | KS | CAA16177, S. coelicolor A3(2), 99/99 | ||
| 3–380c | 81 | ACP | CAA16178, S. coelicolor A3(2), 99/100 | ||
| 3–378 | 87 | ACP | CAA16180, S. coelicolor A3(2), 100/100 | ||
| 3–377 | 641 | ACP/ACP/AMT | CAA16181, S. coelicolor A3(2), 97/97 | ||
| 3–376 | 532 | A(cys) | CAA16182, S. coelicolor A3(2), 98/99 | ||
| 3–375 | 2,298 | CoL/T-KS/AT(m)/ACP/AMT |
SDT77027, Streptomyces sp. 2114.2, 96/96 (CAA16183, S. coelicolor A3(2), 95/96) |
||
| 3–373 | 280 | TE | CAA16185, S. coelicolor A3(2), 98/100 | ||
| t1pks-a | coelimycin | 1–2 | 4,563 | KS/AT(m)/ACP-KS/AT(m)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP- |
SDT78409, Streptomyces sp. 2114.2, 93/95 (CAD55506, S. coelicolor A3(2), 98/98) |
| 1-1 | >595 | KS… | CAC22145, S. coelicolor A3(2), 94/97 | ||
| 34-1 | >1,743 | …AT/DH/KR/ACP-KS… | CAC22145, S. coelicolor A3(2), 92/95 | ||
| 36-1 | >907 | …DH/KR/ACP | CAC22145, S. coelicolor A3(2), 91/93 | ||
| 36-2 | >582 | KS… | CAC22144, S. coelicolor A3(2), 96/97 | ||
| t1pks-b | EPA | 7–477 | 2,074 | KS/AT(m)/ACP/KR/DH |
SDS27436, Streptomyces sp. 2114.2, 96/96 (CAB52353, S. coelicolor A3(2), 95/95) |
| 7–476 | 2,240 | KS/AT | CAB52354, S. coelicolor A3(2), 96/97 | ||
| other t1pks(s) | unknown(s) | 6-1 | >1,561 | KS/AT/ACP-KS… | SCE45938, Streptomyces sp. DvalAA-14, 74/80 |
| 35-1 | >933 | …DH/KR/ACP | APD71595, Streptomyces sp. MM3, 54/65 | ||
| 35-2 | 1,622 | KS/AT(mm)/KR/ACP | AJC56296, Streptomyces sp. 769, 52/64 | ||
| 35-3 | >493 | KS… | APD71977, Streptomyces sp. MM3, 69/81 | ||
| 9–576 | >1,388 | …AT(mm)/KR/ACP-TE | SCD97877, Streptomyces sp. DvalAA-14, 66/75 | ||
| 9–571c | 693 | KS/ACP | WP_052397599, Streptomyces sp. NRRL F-5123, 75/81 | ||
| 9–569c | 3,992 | KS/ACP-KS/AT(mm)/DH/KR/ACP-KS/AT(m)/DH/KR/ACP | CDR05500, Streptomyces iranensis, 48/58 | ||
| t2pks-a | gray spore pigment | 10–372 | 423 | KS | CAB45606, S. coelicolor A3(2), 98/99 |
| 10–371 | 424 | KS | CAB45607, S. coelicolor A3(2), 99/99 | ||
| 10–370 | 90 | ACP | CAB45608, S. coelicolor A3(2), 100/100 | ||
| t2pks-b | unknown | 9–560 | 82 | ACP | WP_031518191, Streptomyces sp. NRRL F-5123, 81/89 |
| 9–561 | 421 | KS | WP_031518190, Streptomyces sp. NRRL F-5123, 85/91 | ||
| 9–562 | 421 | KS | WP_033177057, Streptomyces sp. URHA0041, 86/91 | ||
| t3pks-a | THN | 4–335 | 374 | KS |
SDS82518, Streptomyces sp. 2114.2, 96/98 (CAC01488, S. coelicolor A3(2), 96/98) |
| t3pks-b | phenolic acid | 7–351 | 391 | KS | CAC17488, S. coelicolor A3(2), 90/91 |
CDA, calcium-dependent antibiotic; EPA, eicosapentaenoic acid. The other abbreviations are the same as those of Table 2.
aORFs are shown as a combination of scaffold number and ORF number. Incompletely sequenced ORFs are shown in italics, and undetermined domains are shown as “…”.
bParentheses indicate that the closest homolog is not from S. coelicolor A3(2).
cEncoded on the complementary strand.